European Journal of Plant Pathology (2021) 161, 247-252

Vahid Roumi, Kadriye Caglayan, Mona Gazel and Shifang Li (2021)
Tomato chlorosis virus found to infect Cestrum elegans and C. nocturnum in Turkey
European Journal of Plant Pathology 161 (1), 247-252
Abstract: Cestrum species are being used as hedge and ornamental plants in Turkey. In this study, a Cestrum elegans plant showing chlorotic spots, rings and reddening was subjected to high throughput sequencing of small RNAs, in order to clarify its etiology. Results suggested the presence of tomato chlorosis virus (ToCV), which was further confirmed by RT-PCR using two specific primers amplifying RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of the virus. When 25 Cestrum samples were tested using CP primers, one symptomatic C. elegans and four symptomless C. nocturnum were infected by ToCV. The obtained sequences shared 83–99.9% nucleotide identity with ToCV isolates available in the GenBank. Phylogenetic relationship among Turkish tomato isolates of ToCV and those available in the GenBank showed that two Cestrum spp. isolates of ToCV were closely related to Turkish tomato isolates, while the other three clustered with isolates from different countries.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Kadriye Caglayan, Mona Gazel, Shi-Fang Li

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato chlorosis virus	Cestrum (crop)	Turkey