European Journal of Plant Pathology (2019) 154, 555-563

Mami Ebata, Yosuke Matsushita, Masayuki Morimoto and Tomofumi Mochizuki (2019)
Distribution of Chrysanthemum chlorotic mottle viroid in shoot meristem and flower buds of chrysanthemum
European Journal of Plant Pathology 154 (3), 555-563
Abstract: Detailed information on viroid invasion of the shoot apical meristem is important for the efficient production of viroid-free plants by meristem tip culture. Additionally, knowledge on viroid distribution in plant reproductive tissues, especially the embryo, is crucial to assess seed transmission of viroids. In this study, we analyzed histochemically the distribution of chrysanthemum chlorotic mottle viroid (CChMVd) in the shoot meristems and flower buds of CChMVd-infected chrysanthemum plants (cv. 'Okayama-heiwa'). In situ hybridization revealed that CChMVd invaded the shoot apical meristems and leaf primordia in the shoot meristem at a high frequency (ca. 70%). In flower buds, CChMVd was found in the bract, receptacle vascular, petals, ovules, and immature stamens and pistils. Furthermore, we showed that seed transmission of CChMVd occurred at a high frequency when CChMVd-infected plants were used as female parents. Overall, our findings suggest that CChMVd infection of ovules in flower buds is involved in seed transmission of the viroid.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Yosuke Matsushita, Tomofumi Mochizuki

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Chrysanthemum chlorotic mottle viroid	Chrysanthemum (genus)