European Journal of Plant Pathology (2018) 152, 909-919
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The impact of high throughput sequencing on plant health diagnostics
European Journal of Plant Pathology 152 (4), 909-919
Abstract: High throughput sequencing informed diagnostics is revolutionising plant pathology. The application of this technology is most advanced in plant virology, where it is already becoming a front-line diagnostic tool and it is envisaged that for other types of pathogen and pests this will be the case in the near future. However, there are implications to deploying this technology due to a number of technical and scientific challenges. Firstly, interpretation of data and the assessment of plant health risk against a limited baseline of existing knowledge of the presence of pathogens in a given geographic region. Secondly, evidence of causality and the separation of pathogenic from commensal organisms in the sequence data, thirdly, the tension between the generation of a rapid sequence result with the necessary but laborious epidemiological characterisation in support of plant health risk assessment. Finally, the validation and accreditation of methods based on this rapidly evolving technology. These in turn present challenges for plant health policy and regulation. This review discusses the development of this technology, its application in plant health diagnostics, and explores the implications of applying this technology in the plant health setting.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Kris De Jonghe, Adrian Fox, Neil Boonham
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant. |
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