European Journal of Plant Pathology (2010) 128, 243-250
Shesh Kumari, Wilfrida Decraemer and Francesca De Luca (2010)
Molecular characterization of Xiphinema brevicollum (Nematoda: Longidoridae) from the Czech Republic
European Journal of Plant Pathology 128 (2), 243-250
Abstract: Populations of Xiphinema brevicollum occurring in the Czech Republic were described morphologically and molecularly. Published species-specific primer set BL18 and BV3 was used to amplify three populations of X. brevicollum from the Czech Republic. These primers were tested against 9 species of Xiphinema and 11 species of Longidorus. Amplification was also observed for X. inaequale, X. italiae and X. lambertii. Three additional markers, mitochondrial cytochrome c oxidase subunit 1, ribosomal D2/D3 expansion segment of the 28S gene and 18S gene, were amplified and sequenced for X. brevicollum, X. inaequale and X. lambertii belonging to X. americanum-group. Comparison of cox1 sequences of X. brevicollum from the Czech Republic with X. taylori from the Slovak Republic (accession number AM086702) suggested that these populations represent the same species.
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Link to article at publishers website
Database assignments for author(s): Francesca De Luca
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Xiphinema brevicolle | Czech Republic |