European Journal of Plant Pathology (2008) 120, 85-89

R.C. Holeva, D.J.F. Brown, R.H.C. Curtis and R. Neilson (2008)
Selective recovery of the virus-vector trichodorid nematode Paratrichodorus anemones from soil samples by immunomagnetic capture
European Journal of Plant Pathology 120 (1), 85-89
Abstract: Lectins and polyclonal antiserum that bind specifically and reproducibly to the overall surface of Paratrichodorus anemones were identified and bound to monodisperse superparamagnetic particles (Dynabeads) to assess their efficiency as probes for capturing target nematodes from test suspensions. In recovery experiments, while both types of probe isolated nematodes, antibody-coated beads recovered them more efficiently than beads coated with lectins. When immunomagnetic capture was used to isolate P. anemones from mixtures of naturally occurring populations of nematodes extracted in bulk, from soil samples, 80% of the target nematodes were recovered.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Roy Neilson, Derek J.F. Brown, Rosane H.C. Curtis

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Paratrichodorus anemones