European Journal of Plant Pathology (2004) 110, 45-52

M.M. Klerks, J.L. Lindner, D. Vaškova, J. Špak, J.R. Thompson, W. Jelkmann and C.D. Schoen (2004)
Detection and tentative grouping of Strawberry crinkle virus isolates
European Journal of Plant Pathology 110 (1), 45-52
Abstract: A partial sequence of the putative polymerase (L) protein of Strawberry crinkle virus (SCV), genus Cytorhabdovirus, is described. The virus protein was found to be distantly related to the L protein of the rhabdoviruses Northern cereal mosaic virus, Rice yellow stunt virus and Sonchus yellow net virus. Moreover, a tentative grouping of SCV isolates is described, based on phylogenetic analysis of a region enclosing the GDN-motif within the RNA-dependent RNA polymerase gene. A sequence homology of 98% was found for each tentative group, and heterogeneity of at least 11% was observed between both groups. The tentative grouping did not appear to be related to symptomatology or geographical origin of the isolates. Nevertheless, the use of several reverse-transcription (RT)-PCR primer sets, all directed to different regions within the SCV genome, confirmed the presumptive classification into two groups, namely group I (isolates 1554, KG and Post), and group II (isolates 1553, Hb-A1, 37-1 and 37-2). Additionally, the detection of SCV isolates from herbaceous hosts and strawberry plant material was possible through use of a newly developed gel-based RT-PCR and a gel-free AmpliDet RNA assay. Both methods have the potential to provide rapid, sensitive and specific detection of SCV in in vitro propagation material.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Michel M. Klerks, Jeremy R. Thompson, Wilhelm Jelkmann, Josef Spak

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cytorhabdovirus fragariarugosus