European Journal of Plant Pathology (2004) 110, 213-221

D. Vašková, J. Špak, M.M. Klerks, C.D. Schoen, J.R. Thompson and W. Jelkmann (2004)
Real-time NASBA for detection of Strawberry vein banding virus
European Journal of Plant Pathology 110 (2), 213-221
Abstract: An assay for the detection of Strawberry vein banding virus (SVBV) in Fragaria spp. based on nucleic acid sequence based amplification (NASBA) and real-time detection using molecular beacons (real-time NASBA) is described. This assay was compared both with biological indexing, the current method for certification of SVBV, and a newly optimised PCR-based detection method. Performance of the assay was tested on three SVBV isolates in Fragaria indicator plants and 317 field strawberries from five European countries. The assay was shown to be SVBV-specific, testing negative for other common aphid-borne strawberry viruses. The virus was detected in purified total RNA preparations diluted a millionfold, which is an amount equivalent to ~1 ng of fresh material. The real-time NASBA method developed here offers the potential of a fast, sensitive and reliable approach for the routine diagnosis of strawberry stock material.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jeremy R. Thompson, Wilhelm Jelkmann, Josef Spak, Michel M. Klerks

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Caulimovirus venafragariae	Strawberry (Fragaria)	Czech Republic
	Caulimovirus venafragariae	Strawberry (Fragaria)	Netherlands