European Journal of Plant Pathology (2000) 106, 589-592

D. Louro, G.P. Accotto and A.M. Vaira (2000)
Occurrence and diagnosis of Tomato chlorosis virus in Portugal
European Journal of Plant Pathology 106 (6), 589-592
Abstract: Tomato chlorosis virus (ToCV), a new whitefly-transmitted and phloem-limited Crinivirus infecting tomatoes in Europe, is reported for the first time in Portugal. Tomato plants with symptoms of interveinal chlorosis, collected during autumn 1998 and summer and autumn 1999 in Algarve, southern Portugal, were positive in RT-PCR assays using ToCV-specific primers. The amplified 439 bp fragment was sequenced and showed 99% homology with the ToCV sequence in the GenBank database. A digoxigenin-DNA probe was produced and tested in dot-blot with total RNAs extracted from tomato samples. Both the RT-PCR and dot-blot hybridisation procedures enabled rapid and reliable detection of ToCV from field samples.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Gian Paolo Accotto, Diamantina Louro

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato chlorosis virus	Tomato (Solanum lycopersicum)	Portugal (continental)