European Journal of Plant Pathology (1997) 103, 345-355

Peter Bonants, Marjanne Hagenaar-de Weerdt, Marga van Gent-Pelzer, Isabelle Lacourt, David Cooke and Jim Duncan (1997)
Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction
European Journal of Plant Pathology 103 (4), 345-355
Abstract: Phytophthora fragariae Hickman, which causes strawberry red stele and raspberry root rot, is a quarantine organism for which specific and sensitive detection methods are required to test the health of planting material. Sequences of the internal transcribed spacer regions of the ribosomal gene repeat (rDNA) were used to develop primers for P. fragariae in a nested Polymerase Chain Reaction (PCR). The fungus was readily detected in infected but symptomless roots by nested, but not single-round, PCR. It was also detected in infested water samples obtained from the Dutch General Inspection Service by nested PCR. Detection of PCR products was at least 10-fold more sensitive by PCR-ELISA than by conventional visualisation on agarose gels.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): David E.L. Cooke, Peter J. M. Bonants, Marga P.E. van Gent-Pelzer

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
quarantine treatments/regulations/aspects

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytophthora fragariae	Strawberry (Fragaria)