European Journal of Plant Pathology (1995) 101, 561-566

Elaine Ward (1995)
Improved polymerase chain reaction (PCR) detection of Gaeumannomyces graminis including a safeguard against false negatives
European Journal of Plant Pathology 101 (5), 561-566
Abstract: A previously reported method for polymerase chain reaction (PCR) detection of Gaeumannomyces graminis was modified to simplify it, improve its specificity and decrease the possibility of contamination of the assay. The modified method also allowed discrimination between the G. graminis varieties (tritici and avenae) that are pathogenic to wheat and oats respectively and the variety weakly pathogenic to wheat (var. graminis). An internal positive control for the PCR was also added to the test by including a second pair of primers in the reaction. This positive control has wider application in PCR tests for detection of other fungi.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Gaeumannomyces avenae	Oat (Avena sativa)
	Gaeumannomyces tritici	Wheat (Triticum)