Euphytica (2006) 151, 251-261

Pei-Guo Guo, Gui-Hua Bai, Rong-Hua Li, Gregory Shaner and Michael Baum (2006)
Resistance gene analogs associated with Fusarium head blight resistance in wheat
Euphytica 151 (2), 251-261
Abstract: Fusarium head blight (FHB) is one of the most destructive diseases in wheat. Identification of resistance gene analogs (RGAs) may provide candidate genes for cloning of FHB resistance genes and molecular markers for marker-assisted improvement of wheat FHB resistance. To identify potential RGAs associated with FHB resistance in wheat, 18 primer pairs of RGAs were screened between two parents (Ning7840 and Clark) and seven informative RGA primer combinations were analyzed in their recombinant inbred lines (RILs). Five PCR products amplified from three primer combinations showed significant association with FHB resistance, and their sequences are similar to the gene families of RGAs. Three of them (RGA14-310, RGA16-462, RGA18-356) were putatively assigned to chromosome 1AL and explained 12.73%, 5.57% and 5.9% of the phenotypic variation for FHB response in the F₇ population, and 10.37%, 3.37% and 4.53% in F₁₀ population, respectively; suggesting that these RGAs may play a role in enhancing FHB resistance in wheat. Analysis of nucleotide sequence motifs demonstrated that all the RGA markers contain a heat shock factor that initiates the production of heat shock proteins. A sequence tagged site (STS) marker (FHBSTS1A-160) was successfully converted from RGA18-356, and validated in fourteen other cultivars. Significant interaction between the quantitative trait locus (QTL) on 1AL and the QTL on 3BS was detected. The marker FHBSTS1A-160 in combination with markers linked to the major QTL on 3BS could be used in marker-assisted selection (MAS) for enhanced FHB resistance in wheat.
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Link to article at publishers website
Database assignments for author(s): Guihua Bai, Michael Baum

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium graminearum	Wheat (Triticum)