Euphytica (2002) 123, 21-29

Zhiyong Liu, Qixin Sun, Zhongfu Ni, Eviatar Nevo and Tsomin Yang (2002)
Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer
Euphytica 123 (1), 21-29
Abstract: Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC₁F₂ progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used togenerate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers, STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430,Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of ChineseSpring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Qixin Sun

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Blumeria graminis	Wheat (Triticum)