Euphytica (1999) 107, 23-28

Yong-Hee Jeon, Sang-Nag Ahn, Hae-Chune Choi, Tae-Ryong Hahn and Huhn-Pal Moon (1999)
Identification of a RAPD marker linked to a brown planthopper resistance gene in rice
Euphytica 107 (1), 23-28
Abstract: We report the tagging of a brown planthopper (BPH) resistance gene ( Bph-1) in rice using RAPD and RFLP markers. The Korean rice variety 'Gayabyeo' has dominant duplicate genes including Bph-1 conferring resistance to biotype 1 of BPH. Bulked segregant RAPD analysis was employed for rapid identification of DNA markers linked to resistance genes. For tagging these two genes, an F2F3 population from a 'Gayabyeo' × 'Nagdongbyeo' cross was developed and evaluated for BPH resistance. Three bulked DNAs from two groups of homozygous BPH resistant (each for Bph-1 and the other unknown gene) and homozygous susceptible F2 plants were analyzed by RAPD using 140 random oligomers. One primer, OPD-7 yielded a 700-bp fragment that was present in Gayabyeo and resistant F2 plants (homozygous for Bph-1 locus) but absent in Nagdongbyeo and susceptible F2 plants. Cosegregation of this marker with Bph-1 was verified using an F2 population segregating for Bph-1. Chromosomal regions surrounding the Bph-1 were examined with additional RFLP and microsatellite markers on chromosome 12 to define the location of the RAPD marker and Bph-1. Use of this RAPD marker could facilitate early selection of resistant lines for BPH.
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Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Nilaparvata lugens	Rice (Oryza)