Euphytica (1997) 95, 203-207

Sami Doganlar, Anne Frary and Steven D. Tanksley (1997)
Production of interspecific F₁ hybrids, BC₁, BC₂ and BC₃ populations between Lycopersicon esculentum and two accessions of Lycopersicon peruvianum carrying new root-knot nematode resistance genes
Euphytica 95 (2), 203-207
Abstract: Two accessions of Lycopersicon peruvianum Mill. (PI270435, PI126443) carrying Mi-2 and Mi-3, respectively, new root-knot nematode resistance genes, were selected as the male parents for crosses with L. esculentum Mill. in order to produce interspecific hybrids. Crossability barriers between these two distantly related species were circumvented by ovule culture. A total of ten interspecific F₁ hybrid plants were produced. The hybrid nature of the putative F₁ plants was confirmed by a comparison of several morphological characteristics and a PCR-based assay. Eight of ten hybrid plants were backcrossed with L. esculentum to generate a total of 98 BC₁ progeny. Two lines were advanced to the BC₂ and BC₃ levels. Based on these results, ovule culture was found to be an effective method for the production of novel interspecific F₁ and BC₁ plants.
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Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne (genus)	Tomato (Solanum lycopersicum)