Environmental Entomology (1994) 23, 987-991

Joseph E. Huesing, Richard E. Shade and Larry L. Murdock (1994)
Cowpea trypsin inhibitor and resistance to cowpea weevil (Coleoptera: Bruchidae) in cowpea variety 'TVu 2027'
Environmental Entomology 23 (4), 987-991
Abstract: Seeds of cowpea, Vigna unguiculata (L.) Walp, line 'TVu 2027' are moderately resistant to the cowpea weevil, Callosobruchus maculatus (F.). C. maculatus larvae exhibit higher mortality and take longer to develop in 'TVu 2027' than in more susceptible seeds. Claims have been made (Gatehouse, A.M.R., J. A. Gatehouse, P. Dobie, A. M. Kilminster and D. Boulter. 1979. Biochemical basis of insect resistance in Vigna unguiculata. J. Sci. Food Agric. 30: 948-958) that an elevated level of cowpea trypsin inhibitor (CPTI) confers resistance to 'TVu 2027'. To test this hypothesis, we isolated and purified CPTI and evaluated its impact on cowpea weevil using an artificial seed system. CPTI at dietary levels up to 2.0% (wt/wt) caused no significant increase in mortality of cowpea weevils and only slight delays in within-seed developmental times. These results do not support the hypothesis that CPTI is the basis of resistance in 'TVu 2027'.
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Database assignments for author(s): Richard E. Shade, Larry L. Murdock, Joseph E. Huesing

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Callosobruchus maculatus	Stored legumes