Environmental Entomology (1983) 12, 787-791

R.H. Shukle and L.L. Murdock (1983)
Lipoxygenase, trypsin inhibitor, and lectin from soybeans: Effects on larval growth of Manduca sexta (Lepidoptera: Sphingidae)
Environmental Entomology 12 (3), 787-791
Abstract: The effects of lipoxygenase, lectin, and trypsin inhibitor proteins from soybean seed on larval growth of Manduca sexta have been investigated. Soybean lipoxygenase retarded larval growth when incorporated into an artificial diet at a concentration of 4% (wt/vol). In addition, larvae fed the lipoxygenase diet showed increased levels of midgut cytochrome P-450 protein as compared with controls. Linoleic acid hydroperoxide, a product of lipoxygenase activity, also reduced larval growth at the 0.3% level in the diet, and was lethal to all larvae tested at the 1% level. A highly purified preparation of soybean lectin inhibited larval growth when added at the I% level in the diet, and trypsin inhibitor (Kunitz inhibitor) retarded larval growth when added at the 5% level. These results represent the first evidence that lipoxygenases could function as plant defenses against insect attack, and also demonstrate that a highly purified lectin can inhibit the growth of a phytophagous insect.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Larry L. Murdock

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Manduca sexta