Entomological Research (2020) 50, 483-492

Jing-Jing Li, Ming-Hui Jin, Nian-Meng Wang, Qi-Tong Yu, Ze-Yu Shang and Chao-Bin Xue (2020)
Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella
Entomological Research 50 (10), 483-492
Abstract: Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide-resistance mechanism in DBM. The lab-selected (R_h) and field-collected (R_b) flubendiamide-resistant lines of P. xylostella with resistance ratios of 1889.92-fold and 1250.97-fold, respectively, were used, as well as a lab-reared flubendiamide-susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the R_h group and 432 upregulated and 565 downregulated gene messengers in the R_b group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the R_h line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the R_b line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the R_h group and 221 upregulated and 155 downregulated proteins in the R_b group. Further analyses found that three CYP and 11 CYP proteins were over-expressed in R_h and R_b lines, respectively. Four glutathione S-transferase (GST) and four UGTs were over-expressed in R_b line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.
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Link to article at publishers website
Database assignments for author(s): Chao-Bin Xue

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella