Entomologia Experimentalis et Applicata (1998) 88, 49-58

Evelyne Bauer, Tina Trenczek and Silvia Dorn (1998)
Instar-dependent hemocyte changes in Pieris brassicae after parasitization by Cotesia glomerata
Entomologia Experimentalis et Applicata 88 (1), 49-58
Abstract: Cotesia glomerata L. (Hymenoptera: Braconidae) is a parasitoid of early instar larvae of Pieris brassicae L. (Lepidoptera: Pieridae). Late instars of P. brassicae can more often overcome parasitization by hemocytic encapsulation of C. glomerata eggs. Short-term hemocyte responses to parasitization were examined in third and fourth instar larvae of P. brassicae. Total and differential hemocyte counts did not differ between parasitized and unparasitized host larvae. A rapid, but temporary decrease of total hemocyte as well as plasmatocyte numbers was observed immediately after oviposition. Numbers of hemocytes adhering to tissues were shown to be the same in untreated, wounded and parasitized P. brassicae larvae by tracing hemocytes with monoclonal antibodies as markers. The in vitro spreading ability of hemocytes from unparasitized third and fourth instar larvae was lower than that of the last instar's; parasitization, however, had no influence on hemocyte spreading. We therefore suggest that the higher parasitization success of C. glomerata in earlier instars of P. brassicae is mainly due to the low spreading ability of the hemocytes. Abbreviations: ACS - anticoagulant saline; BSA - bovine serum albumin; DABCO - 1,4-diazabicyclo-[2,2,2]-octane; DHC - differential hemocyte count; FITC - fluorescein isothiocyanate; GR - granular cells; LPS - lipopolysaccharide; mAb - monoclonal antibody; OE - oenocytoids; PL - plasmatocytes; PRO - prohemocytes; PS - Pieris saline; PVP - polyvinylpyrrolidone; TBS - tris-buffered saline; THC - total hemocyte count.
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Link to article at publishers website
Database assignments for author(s): Silvia Dorn

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pieris brassicae
Cotesia glomerata (parasitoid)	Pieris brassicae