EPPO Bulletin (2000) 30, 301-304

M.M. Finetti Sialer, G. Parrella, M. Papanice, C. Vovlas and D. Gallitelli (2000)
Biodiversity of viruses infecting tomato in Italy: methods for diagnosis and diversification
EPPO Bulletin 30 (2), 301-304
Abstract: An account is given of strain-specific diagnostic methods currently adopted in Italy to detect alfalfa mosaic alfamovirus (AMV), cucumber mosaic cucumovirus (CMV) and tomato spotted wilt tospovirus (TSWV). These viruses are highly detrimental to tomato crops as the introduction of new virus strains has deeply changed the population structure of resident viruses. RT-PCR coupled with restriction analysis of amplicons proved suitable for differentiating CMV and AMV strains whereas single-stranded conformational polymorphism (SSCP) was used for the identification of TSWV isolates. Application of strain-specific diagnostic methods to phytosanitary inspection of plant propagules is suggested as a powerful tool for timely identification of new virus strains.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Giuseppe Parrella

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus	Tomato (Solanum lycopersicum)	Italy
	Orthotospovirus tomatomaculae	Tomato (Solanum lycopersicum)	Italy
	Alfalfa mosaic virus	Tomato (Solanum lycopersicum)	Italy