Current Microbiology (2002) 45, 221-225
Zhiming Yuan, Bjarne M. Hansen, Lars Andrup and Jørgen Eilenberg (2002)
Detection of enterotoxin genes in mosquito-larvicidal Bacillus species
Current Microbiology 45 (3), 221-225
Abstract: The presence of the B component of hemolysin BL (hblA), enterotoxin BceT (bceT), and enterotoxin S (entS) genes in mosquito-larvicidal Bacillus sp., including 25 B. sphaericus and 4 B. thuringiensis subsp. israelensis strains, has been analyzed by multiplex PCR in this study. The results showed that all four B. thuringiensis strains contain the hblA gene and the sequences of bceT and entS genes. However, none of the enterotoxin gene sequences were detected in the B. sphaericus strains. The enterotoxin production in all strains has also been analyzed by using two commercial immunoassay kits (TECRA and RPLA), and it has been proved that all the B. thuringiensis strains and one B. cereus strain can produce enterotoxins during growth. No enterotoxin activity could be detected in B. sphaericus strains.
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Link to article at publishers website
Database assignments for author(s): Zhi Ming Yuan, Jørgen Eilenberg, Bjarne Munk Hansen
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Lysinibacillus sphaericus (entomopathogen) | ||||
Bacillus thuringiensis var. israelensis (entomopathogen) |