Canadian Journal of Plant Pathology (2011) 33, 127-134

Yaima Arocha-Rosete, Sara Zunnoon-Khan, Iryna Krukovets, William Crosby, James Scott, Assunta Bertaccini and Roberto Michelutti (2011)
Identification and molecular characterization of the phytoplasma associated with peach rosette-like disease at the Canadian Clonal Genebank based on the 16S rRNA gene analysis
Canadian Journal of Plant Pathology 33 (2), 127-134
Abstract: Peach trees exhibiting peach rosette-like disease symptoms and infected by a phytoplasma of group 16SrI `Candidatus Phytoplasma asteris' at the Canadian Clonal Genebank were further tested for the pathogen characterization based on the 16S rRNA gene. Nested PCR with phytoplasma universal primers R16mF2/R1 and R16F2n/R2 resulted in amplification of products of approximately 1.25 kb from all four symptomatic trees tested. Virtual RFLP of the R16F2n/R2 sequenced amplicons with selected restriction endonucleases showed unique RFLP patterns when compared to the described 16SrI phytoplasma subgroups; these data were confirmed by phylogenetic analyses. The phytoplasma was therefore assigned as a member of a new 16SrI subgroup (16SrI-W). Results represent the first report of a new phytoplasma 16SrI subgroup infecting peach in Canada, and provide a valuable tool for further epidemiological studies on this phytoplasma in peach.
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Link to article at publishers website
Database assignments for author(s): Assunta Bertaccini, Roberto Michelutti

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	16SrI phytoplasma group	Peach/nectarine (Prunus persica)