Canadian Journal of Plant Pathology (2007) 29, 311-316
V. Chandel, T. Rana, V. Hallan and A.A. Zaidi (2007)
Evidence for the occurrence of Prunus necrotic ringspot virus on peach in India by serological and molecular methods
Canadian Journal of Plant Pathology 29 (3), 311-316
Abstract: Prunus necrotic ringspot virus (PNRSV) is a widespread virus that causes tremendous losses on stone fruits. Necrotic symptoms typical of the virus were observed on the leaves of peach trees, Prunus persica 'Elberta', during a survey of the Kullu area of Himachal Pradesh, a northern state of India. PNRSV-specific antibodies were used for serological detection, and positive results were obtained. Molecular detection of the coat-protein (CP) gene of the PNRSV by means of reverse transcription - polymerase chain, with a primer pair that amplifies the complete CP region, amplified the ~700-bp product, which showed a maximum of 98% identity with an isolate from the United States. Multiple alignment of the CP sequence with that of other PNRSV isolates showed 87%-100% identity at the nucleotide and amino acid level.
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Database assignments for author(s): Aijaz Asghar Zaidi, Vanita Chandel
Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Prunus necrotic ringspot virus | Peach/nectarine (Prunus persica) | India |