Canadian Journal of Plant Pathology (2004) 26, 365-370

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N. Allain-Boulé, C.A. Lévesque, C. Martinez, R.R. Bélanger and R.J. Tweddell (2004)
Identification of Pythium species associated with cavity-spot lesions on carrots in eastern Quebec
Canadian Journal of Plant Pathology 26 (3), 365-370
Abstract: In this study, 248 isolates of Pythium spp. were recovered from cavity-spot lesions of carrot and identified by DNA sequencing. The isolates came from carrots harvested in different fields in the area of the city of Québec. The majority of the isolates belonged to Pythium sulcatum and P. sylvaticum, which represented 93% of the isolates collected. Pythium macrosporum, P. debaryanum, P. dissotocum, P. attrantheridium, P. rostratum, and P. acanthicum were also found to be associated with cavity-spot lesions. Among these species, only P. acanthicum failed to cause cavity-spot lesions when inoculated on carrots. Based on mycelial growth, 3%, 40%, 80%, and 100% of P. sylvaticum, P. sulcatum, P. macrosporum, and P. dissotocum isolates, respectively, were tolerant to metalaxyl while all isolates of P. attrantheridium, P. debaryanum, P. rostratum, and P. acanthicum were sensitive. In the area of the city of Québec, P. sulcatum and P. sylvaticum were by far the two most important species associated with the disease. This reinforces the concept that Pythium spp. causing cavity spots in carrot are diverse and may vary according to geographical areas.

Dans la présente étude, 248 isolats de Pythium spp. furent isolés de lésions de la maladie de la tache de la carotte et identifiés par séquençage de l'ADN. Les isolats provenaient de carottes récoltées dans différents champs de la région de Québec. La majorité des isolats appartenaient au Pythium sulcatum et au P. sylvaticum qui représentaient 93% des isolats obtenus. Il fut constaté que le Pythium macrosporum, le P. debaryanum, le P. dissotocum, le P. attrantheridium, le P. rostratum et le P. acanthicum étaient aussi associés aux lésions de la maladie de la tache. Parmi ces espèces, seul le P. acanthicum n'a pas réussi à causer la maladie de la tache lorsque inoculé à des carottes. En fonction de la croissance mycélienne, respectivement 3%, 40%, 80% et 100% des isolats de P. sylvaticum, P. sulcatum, P. macrosporum et de P. dissotocum furent tolérants au métalaxyl, alors que tous les isolats de P. attrantheridium, P. debaryanum, P. rostratum et P. acanthicum y furent sensibles. Dans la région de Québec, le P. sulcatum et le P. sylvaticum furent de loin les deux plus importantes espèces associées à la maladie. Cette constatation appuie l'idée que diverses espèces de Pythium causent la maladie de la tache chez la carotte et qu'elles peuvent varier selon les régions géographiques.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Richard R. Belanger, C. André Lévesque

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Globisporangium macrosporum Carrot (Daucus carota) Canada (east)
Pythium sulcatum Carrot (Daucus carota) Canada (east)
Pythium dissotocum Carrot (Daucus carota) Canada (east)
Globisporangium rostratum Carrot (Daucus carota) Canada (east)
Globisporangium sylvaticum Carrot (Daucus carota) Canada (east)
Globisporangium debaryanum Carrot (Daucus carota) Canada (east)
Globisporangium attrantheridium Carrot (Daucus carota) Canada (east)