Canadian Journal of Microbiology - Revue Canadienne de Microbiologie (1997) 43, 1069-1073

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Maureen O'Callaghan, Trevor A. Jackson and Travis R. Glare (1997)
Serratia entomophila bacteriophages: host range determination and preliminary characterization
Canadian Journal of Microbiology - Revue Canadienne de Microbiologie 43 (11), 1069-1073
Abstract: Eight bacteriophages specific to Serratia entomophila, a commercially available bacterial pathogen of the New Zealand grass grub (Costelytra zealandica), were characterized by host range determination, morphology and restriction endonuclease patterns of DNA. Phages were originally isolated from grass grub larvae and fermenter broth where phages had disrupted large-scale production of S. entomophila. Seven of the phages (CW1l -CW5, BC, and BT) had heads similar in size (approximately 60 x 60 nm) and long noncontractile tails (185 x 10 nm). Phage AgRP8 (P8) had a smaller head and a short tail structure. Restriction endonuclease analysis divided the phages into four groups: CW2, CW4, CW5, BC, and BT gave identical patterns, while CW 1, CW3, and P8 each gave different patterns. Six distinct phage groups were distinguished by host range determination, after screening phages against 70 bacterial isolates: CW 1, CW2/CW4, CW3, CW5, BC/BT, and P8. While confirming the indicated groupings by DNA analysis, it was possible to distinguish between some of the phages in the largest group: CW2/4 could be distinguished from CW5 and BC/BT. Screening of soil bacterial isolates of S. entomophila against nondiluted phages will aid in monitoring the establishment and persistence of strains applied for biological control of the grass grub.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Travis R. Glare, Maureen O'Callaghan

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Costelytra giveni New Zealand
Serratia entomophila (entomopathogen) Costelytra giveni New Zealand
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