Canadian Journal of Botany - Revue Canadienne de Botanique (1994) 72, 440-447

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Hanhong Bae, Everett M. Hansen and Steven H. Strauss (1994)
Restriction fragment length polymorphisms demonstrate single origin of infection centers in Phellinus weirii
Canadian Journal of Botany - Revue Canadienne de Botanique 72 (4), 440-447
Abstract: Restriction fragment length polymorphism (RFLP) markers were used to study genetic variation in the basidiomycete fungus Phellinus weirii (Murr.) Gilbertson, the cause of laminated root rot of conifers. In an initial study, three isolates each from the Douglas-fir type and the cedar-type biological species were surveyed with 12 restriction enzymes and 20 random, mitochondrial, and nuclear-ribosomal gene probes. The two biological species were distinct with most probe-enzyme combinations (91%). Variation within biological species was detected for the random and ribosomal DNA probes but not for the mitochondrial DNA probes. In a subsequent study 65 probe-enzyme combinations (13 × 5) that had detected variation within the Douglas-fir type biological species were used to analyze 27 isolates derived from six infection centers, two host species, and two geographic areas in western Oregon. Infection centers differed from one another in numerous probe-enzyme combinations but were nearly genetically uniform within. Isolates from the two host species, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and mountain hemlock (Tsuga mertensiana (Bong.) Carr.), showed few RFLP differences. Initiation of infection centers, and subsequent vegetative or basdiospore initiated immigration, appear to be rare events.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Everett M. Hansen

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Coniferiporia weirii Hemlock (Tsuga) U.S.A. (NW)
Coniferiporia weirii Douglas fir (Pseudotsuga menziesii) U.S.A. (NW)
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