Bulletin of Insectology (2011) 64, S59-S60

Mohammad Salehi, Keramatollah Izadpanah, Majid Siampour and Seyyed A. Esmailzadeh-Hosseini (2011)
Polyclonal antibodies for the detection and identification of Fars alfalfa witches' broom phytoplasma
Bulletin of Insectology 64 (Suppl.), S59-S60
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: Alfalfa witches' broom (AlWB) is a destructive phytoplasma disease in Iranian southern and central provinces. Antiserum was raised by injection of partially purified Fars AlWB phytoplasma (FAlWBP) from 20 g infected alfalfa tissue into a rabbit. The specificity of the polyclonal antibody against phytoplasmas was shown by its reaction with diseased, but not with healthy alfalfa upon dot immunobinding assay (DIBA) tests. Phytoplasmas associated with Borazjan fababean phyllody and Chahbahar periwinkle phyllody were also shown to be serologically related to FAWBP. No serological relationship was found between FAlWBP and phytoplasmas associated with Yazd AlWB, witches' broom disease of lime, Khafr almond witches' broom, carrot witches' broom, sesame phyllody, eggplant big bud and tomato big bud.
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Database assignments for author(s): Mohammad Salehi, Keramat Izadpanah

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	16SrII phytoplasma group	Alfalfa/lucerne (Medicago sativa)	Iran