Bulletin of Insectology (2011) 64, S17-S18

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Jelena Mitrovic, Nicoletta Contaldo, Samanta Paltrinieri, Juan F. Mejia, Nicola Mori, Assunta Bertaccini and Bojan Duduk (2011)
The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples
Bulletin of Insectology 64 (Suppl.), S17-S18
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of `Candidatus Phytoplasma asteris' in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation among strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.
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Database assignments for author(s): Bojan Duduk, Nicoletta Contaldo, Assunta Bertaccini

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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Phytoplasma asteris Cabbage (Brassica oleracea) Italy
Phytoplasma asteris Carrot (Daucus carota) Serbia
Phytoplasma asteris Maize/corn (Zea mays) Colombia
Phytoplasma asteris Rape/canola (Brassica napus) Italy
Phytoplasma asteris Lettuce (Lactuca) Italy
Phytoplasma asteris Onion/garlic/leek (Allium) Italy
Phytoplasma asteris Poplar/aspen (Populus) Serbia
Phytoplasma asteris Oil palm (Elaeis guineensis) Colombia