Bulletin of Insectology (2007) 60, 253-254

S. Palmano, P. Margaria, C. Marzachì and M. Turina (2007)
Detection of 'flavescence dorée' in grapevines by RT-PCR
Bulletin of Insectology 60 (2), 253-254
First International Phytoplasmologist Working Group Meeting (IPWG), Bologna (Italy) November 12-15, 2007
Abstract: A rapid protocol for extracting crude sap from grapevine samples, and successive one-tube amplification by reverse transcriptase PCR (RT-PCR), was applied on symptomatic and symptomless plants to detect the presence of 'flavescence dorée' phytoplasma. Results were compared with those obtained by utilizing nested-PCR on phytoplasma DNA-enriched preparations. Statistical analyses showed a good correlation between the two sets of results, with advantages for the RT-PCR as being rapid and reliable when used for large scale screening.
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Database assignments for author(s): Sabrina Palmano, Massimo Turina, Paolo Margaria, Cristina Marzachi

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma vitis	Grapevine (Vitis)	Italy