Bulletin of Insectology (2007) 60, 223-224

R. D'Amelio, C. Marzachì and D. Bosco (2007)
Double infection of `Candidatus Phytoplasma asteris' and 'flavescence dorée' phytoplasma in the vector Euscelidius variegatus
Bulletin of Insectology 60 (2), 223-224
First International Phytoplasmologist Working Group Meeting (IPWG), Bologna (Italy) November 12-15, 2007
Abstract: Competition between chrysanthemum yellows (CY) and "flavescence dorée" (FD70) phytoplasmas was studied. In the experiments the insect vector Euscelidius variegatus was allowed to acquire both phytoplasmas by feeding first on CY and then on FD70 infected plants and viceversa. Titre of both phytoplasmas was quantified over time in the same insects. Transmission experiments to broad bean, host-plant of both phytoplasmas, were carried out from 14 days after the start of the acquisition until death of leafhoppers. Quantitative Real Time PCR assays demonstrated that FD70 multiplies at a higher rate in E. variegatus compared to CY. Both phytoplasmas reached similar titres in single- and double-infected insects. CY titre increases faster when the vector already acquired FD70. In spite of its lower multiplication in the insect body, CY is much more efficiently transmitted than FD70 by double-infected insects.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Domenico Bosco, Cristina Marzachi

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma vitis
	Phytoplasma asteris
	Euscelidius variegatus