Biocontrol Science and Technology (2021) 31, 1283-1297

Jairo Cristóbal-Alejo, Abril Diaz-Braga, Elizabeth Herrera-Parra, Gabriela Heredia, Irma L. Medina-Baizabal, Blondy Canto-Canche, Miguel Tzec-Sima and Marcela Gamboa-Angulo (2021)
Clonostachys rosea selected by nematicidal screening and its efficacy against Meloidogyne incognita in a greenhouse
Biocontrol Science and Technology 31 (12), 1283-1297
Abstract: Meloidogyne incognita, one of the most important root-knot nematodes of crops, is still mainly controlled with synthetic agrochemicals. Toward finding eco-friendly alternative controls such as biocontrol fungi or fungal extracts, here fungi were screened in vitro for nematicidal potential, then evaluated for efficacy against M. incognita in greenhouse tomato plants. Forty fungal extracts were also tested at 300 µg mL^-1 against second-stage juveniles of M. incognita. Then the strain that produced the most active extract was added (six treatments, mycelium/conidia) to nematode-infested soil in a pot with tomato plants. Agronomic and reproductive variables were measured after 92 days. The best nematicidal extract was from Clonostachys rosea TH27 (LD₅₀ = 375 µg mL^-1), which reduced the number of galls, eggs and females in tomato roots and slightly improved agronomic traits compared with untreated plants. For control of M. incognita in pots in protected conditions, 2 g of inoculum per pot (5 kg) is recommended. Our findings confirmed that C. rosea mycelia has potential for control of M. incognita in tomato plants in the greenhouse.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne incognita	Tomato (Solanum lycopersicum)	Mexico
Clonostachys rosea (antagonist)	Meloidogyne incognita	Tomato (Solanum lycopersicum)	Mexico