Biocontrol Science and Technology (2009) 19, 999-1005

Steven J. Rauth and Ruth A. Hufbauer (2009)
PCR-RFLP assays for discerning three weevil stem feeders (Ceutorhynchus spp.) (Col.: Curculionidae) on garlic mustard (Alliaria petiolata)
Biocontrol Science and Technology 19 (9), 999-1005
Abstract: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were developed to rapidly and cost-effectively differentiate the morphologically indistinguishable larval stages of three species, Ceutorhynchus alliariae, C. roberti and C. scrobicollis, proposed for the biological control of Alliaria petiolata. A PCR-RFLP using Taq-alpha-I restriction enzyme can differentiate the three target species only, while a PCR-RFLP double digestion using the restriction enzymes AluI and Taq-alpha-I should enable, based on virtual digestion, distinguishing them from 12 other Ceutorhynchus species associated with A. petiolata.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ruth A. Hufbauer

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Alliaria petiolata (weed)
Ceutorhynchus alliariae (weed bioagent)
Ceutorhynchus scrobicollis (weed bioagent)
Ceutorhynchus roberti (weed bioagent)