Biocontrol Science and Technology (2002) 12, 427-441

Birgit Jensen, Inge M.B. Knudsen and Dan Funck Jensen (2002)
Survival of conidia of Clonostachys rosea on stored barley seeds and their biocontrol efficacy against seed-borne Bipolaris sorokiniana
Biocontrol Science and Technology 12 (4), 427-441
Abstract: Survival and biocontrol activity of Clonostachys rosea (isolate IK726) conidia during storage on barley seeds were investigated. The initial density of colony forming conidia on seed was 4 2 10³ to 9 2 10⁴ colony forming units (cfu)/seed. After 5 months storage at 4°C, the density decreased by less than one order of magnitude and the biocontrol efficacy against seedling blight caused by seed-borne Bipolaris sorokiniana was maintained at a significantly high level ( > 80% disease reduction) for > 5 months. Conidial survival on seeds stored at 20°C declined more rapidly than at 4°C, and biocontrol efficacy was significantly reduced after 3-5 months. However, conidia produced on solid media over 20 days survived better than conidia produced in liquid culture and conidia from solid media produced over 12 days. In contrast, when seeds treated with conidia were packed with silica gel and stored at 20°C, the cfu density decreased by less than one order of magnitude after 5 months and the biocontrol efficacy was still high after 6 months. A dose-response curve revealed that 103 cfu/seed were needed for 80% control of seedling blight. Similar control was obtained in storage experiments when approximately 103 cfu/seed were recovered from seed, indicating that conidia which survived also retained a high ability to control disease.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Birgit Jensen, Dan Funck Jensen

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
application technology
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bipolaris sorokiniana	Barley (Hordeum vulgare)
Clonostachys rosea (antagonist)	Bipolaris sorokiniana	Barley (Hordeum vulgare)