BMC Plant Biology (2014) 14 (211) - Induction of innate immune ...

Guixia Hao, Marco Pitino, Fang Ding, Hong Lin, Ed Stover and Yongping Duan (2014)
Induction of innate immune responses by flagellin from the intracellular bacterium, ' Candidatus Liberibacter solanacearum'
BMC Plant Biology 14 (211)
Abstract:
Background
' Candidatus Liberibacter solanacearum' (Lso) is a phloem-limited alphaproteobacterium associated with the devastating zebra chip disease of potato (Solanum tuberosum). Like other members of Liberibacter, Lso-ZC1 encodes a flagellin domain-containing protein (Fla _Lso ) with a conserved 22 amino-acid peptide (flg22 _Lso ). To understand the innate immune responses triggered by this unculturable intracellular bacterium, we studied the pathogen-associated molecular patterns (PAMPs) that triggered immunity in Nicotiana benthamiana, using the flg22 _Lso peptide and the full length fla _Lso gene.
Results
Our results showed that the expression of fla _Lso via Agrobacterium-mediated transient expression induced a slow necrotic cell death in the inoculated leaves of N. benthamiana, which was coupled with a burst of reactive oxygen species (ROS) production. Moreover, the expression of several representative genes involved in innate immunity was transiently up-regulated by the flg22 _Lso in N. benthamiana. The Fla _Lso , however, induced stronger up-regulation of these representative genes compared to the flg22 _Lso , especially that of flagellin receptor FLAGELLIN SENSING2 (FLS2) and respiratory burst oxidase (RbohB) in N. benthamiana. Although neither cell death nor ROS were induced by the synthetic flg22 _Lso , a weak callose deposition was observed in infiltrated leaves of tobacco, tomato, and potato plants.
Conclusion
The flagellin of Lso and its functional domain, flg22 _Lso share characteristics of pathogen-associated molecular patterns, and trigger unique innate immune responses in N. benthamiana. Slow and weak activation of the innate immune response in host plants by the flagellin of Lso may reflect the nature of its intracellular life cycle. Our findings provide new insights into the role of the Lso flagellin in the development of potato zebra chip disease and potential application in breeding for resistance.
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Database assignments for author(s): Hong Lin

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Liberibacter solanacearum	Potato (Solanum tuberosum)