Australian Journal of Entomology (2009) 48, 65-72

Martin O. McLoon and Grant A. Herron (2009)
PCR detection of pirimicarb resistance in Australian field isolates of Aphis gossypii Glover (Aphididae: Hemiptera)
Australian Journal of Entomology 48 (1), 65-72
Abstract: Aphis gossypii Glover (cotton aphid) is a major secondary pest of Australian cotton that readily develops resistance to the carbamate insecticide pirimicarb (Pirimor®) and to organophosphates generally. To test the pirimicarb resistance status of Australian strains of A. gossypii, a polymerase chain reaction (PCR) assay followed by restriction enzyme assay (REA) was designed to identify the AceI polymorphism S431F known to be responsible for resistance. The method was tested against reference and 33 field strains collected over two consecutive seasons. Both methods confirmed pirimicarb resistance in two field strains, one from each cotton season, giving credence to the molecular technique described. The PCR assay proved specific for the AceI gene. This PCR REA assay has the potential to replace bioassay for the routine pirimicarb resistance monitoring in A. gossypii. With the molecular assay providing results in 48 h, compared with 4-8 weeks for bioassay, such an assay could be used before insecticide control.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Grant A. Herron

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis gossypii	Cotton (Gossypium)