Australian Journal of Entomology (1998) 37, 174-179

W. Amornsak, G. Gordh and G. Graham (1998)
Detecting parasitised eggs with polymerase chain reaction and DNA sequence of Trichogramma australicum Girault (Hymenoptera : Trichogrammatidae)
Australian Journal of Entomology 37 (2), 174-179
Abstract: The nuclear internal transcribed spacer 2 (ITS2) regions of the egg parasitoid Trichogramma australicum Girault, cotton bollworm Helicoverpa armigera (Hübner) and Helicoverpa punctigera (Wallengren) were amplified by the polymerase chain reaction (PCR) to yield 408, 588 and 644 b.p. fragments, respectively. DNA sequence information was used to design unique primers for specific amplification of T. australicum DNA from individual parasitised eggs. Helicoverpa australicum-specific PCR products were first visible 12 h after eggs were parasitised. This PCR assay can be used to monitor wasp populations under field conditions.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
surveys/distribution/isolation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Trichogramma australicum (parasitoid)