Australasian Plant Pathology (2015) 44, 567-574

Soheila Talesh-Sasani, Bahram Mohammad Soltani, Mehrdad Behmanesh and Naser Safaie (2015)
A Magnaporthe Avr-pita gene orthologous in Rhizoctonia solani AG1-IA shows characteristics of an effector protein
Australasian Plant Pathology 44 (5), 567-574
Abstract: Sheath blight is one of the most destructive diseases of rice that is caused by Rhizoctonia solani. Therefore, understanding molecular mechanisms of rice–Rhizoctonia interaction is necessary for adoption of effective approaches of disease management. Here for the first time, we report an ortholog of Magnaporthe oryzae Avr _- Pita1 gene, designated as Rhiz_Pita in R. solani AG1-IA. Rhiz_Pita showed multiple characteristics of effector proteins; First, more than 98 % identity was calculated between Rhiz_Pita deduced protein sequence and Mag-Pita complete protein sequence. SignalP and PrediSi programs predicted a secretion signal upstream of deduced ORF of Rhiz _- Pita gene. Also, Rhiz_Pita secretion potential was approved by using yeast secretion trap system (YST). Detected by Real-time PCR, Rhiz _- Pita gene expression level was increased following the application of rice leaf extraction in R. solani culture medium. Also, Rhiz _{_} Pita gene expression was significantly elevated in the infected rice plants since 24 h post infection. Overall, these results suggest that novel Rhiz _- Pita gene might encode a bona fide effector protein that its expression is associated to the fungal penetration in the early stages of infection.
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Link to article at publishers website
Database assignments for author(s): Naser Safaie

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Rhizoctonia solani