Australasian Plant Pathology (2013) 42, 73-78

Yiping Hou, Qingquan Luo, Changjun Chen and Mingguo Zhou (2013)
Application of tetra primer ARMS-PCR approach for detection of Fusarium graminearum genotypes with resistance to carbendazim
Australasian Plant Pathology 42 (1), 73-78
Abstract: Tetra-primer ARMS-PCR (amplification refractory mutation system-PCR) was developed to detect isolates of Fusarium graminearum with resistance to carbendazim, a methyl benzimidazole carbamate-group fungicide. Resistance to carbendazim in F. graminearum is caused by point mutations at codon 167, 198, and 200 of the β2-tubulin gene (FGSG_06611.3). According to single nucleotide polymorphisms on codon 167, 198, 200 of the resistance gene β2-tubulin gene, two pairs of primer designed on each codon respectively, were used for amplification. SNP genotyping was made successfully based on the different patterns in agarose gels between point mutations and wild type strain. Tetra-primer ARMS-PCR protocol developed in this study is a simple and useful technique to identify genotypes and has a potential application in management of carbendazim resistance in F. graminearum. It will help researchers and growers select the best antifungal strategy to use in the field.
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Link to article at publishers website
Database assignments for author(s): Ming-Guo Zhou, Chang-Jun Chen, Yiping Hou

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium graminearum