Australasian Plant Pathology (2009) 38, 216-221

J. Sudisha, S. Ananda Kumar, S.R. Niranjana, N.P. Shetty and H. Shekar Shetty (2009)
Cloning and development of pathotype-specific SCAR marker associated with Sclerospora graminicola isolates from pearl millet
Australasian Plant Pathology 38 (3), 216-221
Abstract: Downy mildew pathogen of pearl millet in India is associated with the spread of the highly virulent Sclerospora graminicola pathotype-1. Twenty-seven S. graminicola isolates were screened using 20 inter simple sequence repeats (ISSR). Dinucleotide repeat primer [17898A-(CA)₆ AC] amplified a ~600 bp fragment specific to five isolates of pathotype-1 (Sg 048, Sg 153, Sg 212, DM-11 and DM-90). The ISSR fragment linked with pathotype-1 was cloned successfully and sequenced. To convert ISSR fragments into pathotype-specific sequence characterised amplified region (SCAR) markers, PCR primers were designed using a sequence of the cloned DNA fragment. PCR amplification using SCAR primer pair (UOM3-Sg-Path1-F/R) amplified a single 284 bp band only in isolates of S. graminicola pathotype-1. This SCAR primer pair did not amplify the 284 bp product from the other five S. graminicola pathotypes or a negative control, which demonstrates primer specificity for pathotype-1. The SCAR primer pair (UOM3-Sg-Path1-F/R) obtained in this study will provide a valuable tool for rapid identification and specific detection of S. graminicola pathotype-1.
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Link to article at publishers website
Database assignments for author(s): Hunthrike Shekar Shetty, Jogaiah Sudisha, Siddapura Ramachandrappa Niranjana

Research topic(s) for pests/diseases/weeds:
population dynamics/ epidemiology
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sclerospora graminicola	Pearl millet (Cenchrus americanus)	India