Australasian Plant Pathology (2001) 30, 37-44

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K.J. Williams, C. Smyl, A. Lichon, K.Y. Wong and H. Wallwork (2001)
Development and use of an assay based on the polymerase chain reaction that differentiates the pathogens causing spot form and net form of net blotch of barley
Australasian Plant Pathology 30 (1), 37-44
Abstract: Two forms of barley net blotch are caused by different formae of the fungus Pyrenophora teres and both are economically important pathogens. The spot form of the net blotch fungus (P. teres f. maculata) and the net form of the net blotch fungus (P. teres f. teres) cause the lesion types indicated by their disease names, although symptom overlap and similar spore morphology can make identification difficult. Randomly amplified polymorphic DNA fragments differentiated the two forms of Pyrenophora. Polymorphic bands were cloned and sequenced to develop specific primer sets. A simple assay based on the polymerase chain reaction was developed and can identify the Pyrenophora formae causing disease symptoms directly from infected plant tissues in a single multiplex reaction. The assay was validated using amplified fragment length polymorphism genotyping of isolates and was shown to be more accurate than reliance on symptom expression. This assay can now be used for routine diagnosis, epidemiological studies and resistance breeding, where correct identification of each pathogen is critical.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Hugh Wallwork

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
resistance/tolerance/defence of host


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Pyrenophora teres Barley (Hordeum vulgare)