Australasian Plant Pathology (1996) 25, 234-239

S. Simpfendorfer, T.J. Harden and G.M. Murray (1996)
Viability and pathogenicity of Phytophthora clandestina after storage in water and liquid nitrogen
Australasian Plant Pathology 25 (4), 234-239
Abstract: Survival, pathogenicity, and in vitro growth rate of three isolates of Phytophthora clandestina were tested at monthly intervals for up to 10 months following storage in liquid nitrogen and sterile deionised water on millet seeds at 44°C. Storage of cultures in sterile deionised water on millet seeds maintained high recovery rates (75 - 80%) of all three isolates after 10 months without loss of pathogenicity. The success of liquid nitrogen storage of P. clandestina was variable with only one isolate surviving more than 3 months storage. There was also an effect of the protective solutions used on long term storage and maintenance of pathogenicity. Glycerol (10%) allowed a high recovery rate (89%) of one isolate without loss of pathogenicity for 10 months whilst DMSO (5%) provided a maximum storage period of 3 months with a significant reduction in pathogenicity in the three isolates tested. This study demonstrates that sterile water storage is superior to liquid nitrogen for the medium to long term storage of P. clandestina.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytophthora clandestina