Archives of Virology (2014) 159, 811-815

R. Souza Richards, I.P. Adams, J.F. Kreuze, J. De Souza, W. Cuellar, A.M. Dullemans, R.A.A. Van Der Vlugt, R. Glover, U. Hany, M. Dickinson and N. Boonham (2014)
The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses
Archives of Virology 159 (4), 811-815
Abstract: The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jan F. Kreuze, Wilmer J. Cuellar, René A.A. van der Vlugt, Rachel H. Glover, Neil Boonham, Ummey Hany

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Nepovirus solani	Potato (Solanum tuberosum)