Archives of Virology (2014) 159, 1187-1192

M. Rossi, A. Genre and M. Turina (2014)
Genetic dissection of a putative nucleolar localization signal in the coat protein of Ourmia melon virus
Archives of Virology 159 (5), 1187-1192
Abstract: Ourmiaviruses became the object of recent attention for their unusual taxonomic placements among plant viruses. The ourmia melon virus (OuMV) RNA3 encodes a 22-kDa coat protein (CP). Besides its role in virion formation, the OuMV CP facilitates systemic virus spread. In Nicotiana benthamiana, an eGFP-CP fusion protein was localized in the nucleus and preferentially in the nucleolus. By bioinformatics analysis, we identified an arginine- and lysine-rich region at the N-terminus of the CP. Here, we demonstrate by deletion and alanine scanning mutagenesis that this region in the CP is responsible for its preferential accumulation in the nucleolus of host cells.
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Link to article at publishers website
Database assignments for author(s): Massimo Turina

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ourmia melon virus