Archives of Virology (2012) 157, 1137-1142

Belén Márquez-Martín, Diego Maeso, Ada Martínez-Ayala, Roberto Bernal, M. Teresa Federici, Paul Vincelli, Jesús Navas-Castillo and Enrique Moriones (2012)
Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay
Archives of Virology 157 (6), 1137-1142
Abstract: Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jesús Navas-Castillo, Paul Vincelli

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato rugose yellow leaf curl virus	Tomato (Solanum lycopersicum)	Uruguay