Archives of Virology (2010) 155, 423-428

Afshin Hassani-Mehraban, Marleen Botermans, J.Th.J. Verhoeven, Ellis Meekes, Janneke Saaijer, Dick Peters, Rob Goldbach and Richard Kormelink (2010)
A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia
Archives of Virology 155 (3), 423-428
Abstract: A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5' untranslated region and a part of the intergenic region 3' of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Afshin Hassani-Mehraban, Jacobus Th.J. Verhoeven, Dick Peters

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orthotospovirus alstroemerinecrosis	Alstroemeria (crop)	Colombia