Archives of Virology (2009) 154, 337-341
Olga Zayakina, Marina Arkhipenko, Alexander Smirnov, Nina Rodionova, Olga Karpova and Joseph Atabekov (2009)
Restoration of Potato virus X coat protein capacity for assembly with RNA after His-tag removal
Archives of Virology 154 (2), 337-341
Abstract: We found that the fusion of hexahistidine (H)6 tag to the potato virus X (PVX) coat protein (CP) abolished its ability to be assembled with viral RNA into helical nucleoprotein virus-like particles (VLPs). Instead, irregular agglomerates were produced upon incubation of PVX RNA with (H)6-tagged PVX CP. A factor Xa recognition site, IEGR, was inserted upstream of the CP coding sequence. Removal of the (H)6 tag from PVX CP by Xa protease restored its ability to bind RNA and to assemble VLPs. In addition to the canonical IEGR site, the factor Xa protease was found to cleave PVX CP at a second (non-consensus) site, AVTRGR, located close to the C-terminus of PVX CP. The latter cleavage did not affect reassembly of the PVX RNA and CP into VLPs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Joseph G. Atabekov
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Potato virus X |
