Archives of Virology (2002) 147, 2313-2325

I. Cortez, A. Aires, A.-M. Pereira, R. Goldbach, D. Peters and R. Kormelink (2002)
Genetic organisation of iris yellow spot virus M RNA: indications for functional homology between the G_(C) glycoproteins of tospoviruses and animal-infecting bunyaviruses
Archives of Virology 147 (12), 2313-2325
Abstract: The complete nucleotide sequence (4838 nucleotides) of Iris yellow spot virus (IYSV) M RNA indicates, typical for tospoviruses, the presence of two genes in ambisense arrangement. The vRNA ORF codes for the potential cell-to-cell movement (NSm) protein (34.8 kDa) and the vcRNA ORF for the viral glycoprotein (G1/G2) precursor (128.6 kDa). Multiple sequence alignment of the NSm and G1/G2 precursor proteins of IYSV with those of other tospoviruses, showed highest homologies to Peanut bud necrosis virus (PBNV) and Watermelon silver mottle virus (WSMV). The potential cell-to-cell movement protein of tospoviruses is highly conserved (40-70% identity), with the exception of the first 60 N terminal amino acids, a domain that clearly diverged. For the G1 and G2 viral glycoproteins, blast searches revealed a significant homology between the C-terminally located tospoviral G1 (G_(C)) protein with the counterpart of the animal-infecting bunyaviruses, suggesting a functional homology for these proteins.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Dick Peters

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orthotospovirus iridimaculaflavi