Applied and Environmental Microbiology (2009) 75, 2139-2147
Susan M. Lehman, Andrew M. Kropinski, Alan J. Castle and Antonet M. Svircev (2009)
Complete genome of the broad-host-range Erwinia amylovora phage Phi-Ea21-4 and its relationship to Salmonella Phage Felix O1
Applied and Environmental Microbiology 75 (7), 2139-2147
Abstract: The first complete genome sequence for a myoviridal bacteriophage, Phi-Ea21-4, infecting Erwinia amylovora, Erwinia pyrifoliae, and Pantoea agglomerans strains has been determined. The unique sequence of this terminally redundant, circularly permuted genome is 84,576 bp. The Phi-Ea21-4 genome has a GC content of 43.8% and contains 117 putative protein-coding genes and 26 tRNA genes. Phi-Ea21-4 is the first phage in which a precisely conserved rho-independent terminator has been found dispersed throughout the genome, with 24 copies in all. Also notable in the Phi-Ea21-4 genome are the presence of tRNAs with six- and nine-base anticodon loops, the absence of a small packaging terminase subunit, and the presence of nadV, a principle component of the NAD+ salvage pathway, which has been found in only a few phage genomes to date. Phi-Ea21-4 is the first reported Felix O1-like phage genome; 56% of the predicted Phi-Ea21-4 proteins share homology with those of the Salmonella phage. Apart from this similarity to Felix O1, the Phi-Ea21-4 genome appears to be substantially different, both globally and locally, from previously reported sequences. A total of 43 of the 117 genes are unique to Phi-Ea21-4, and 32 of the Felix O1-like genes do not appear in any phage genome sequences other than Phi-Ea21-4 and Felix O1. N-terminal sequencing and matrix-assisted laser desorption ionization-time of flight analysis resulted in the identification of five Phi-Ea21-4 genes coding for virion structural proteins, including the major capsid protein.
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Link to article at publishers website
Database assignments for author(s): Antonet M. Svircev, Alan J. Castle
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Erwinia amylovora | ||||
| Pantoea agglomerans | ||||
| Erwinia pyrifoliae | ||||
| Erwinia phages (antagonist) | Erwinia amylovora | |||
| Erwinia phages (antagonist) | Pantoea agglomerans | |||
| Erwinia phages (antagonist) | Erwinia pyrifoliae |
