Applied and Environmental Microbiology (2003) 69, 4190-4191

Josie Williams, John M. Clarkson, Peter R. Mills and Richard M. Cooper (2003)
A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost
Applied and Environmental Microbiology 69 (7), 4190-4191
Abstract: We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
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Database assignments for author(s): Richard M. Cooper

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Trichoderma harzianum	Agaricus (crop)