Applied and Environmental Microbiology (1997) 63, 4421-4426

Y.X. Zhang and K. Geider (1997)
Differentiation of Erwinia amylovora strains by pulsed-field gel electrophoresis
Applied and Environmental Microbiology 63 (11), 4421-4426
Abstract: Erwinia amylovora strains, isolated from several host plants in various geographic regions during different years, were analyzed by pulsed- field gel electrophoresis (PFGE) after digestion of the DNA from lysed, agar-embedded cells with rare-cutting restriction enzymes. The banding patterns obtained with enzyme XbaI digests revealed significant differences among strains from different areas. North American strains E9 and Ea-Rb, a Rubus strain, were highly divergent from other E. amylovora strains. French strains were different from central European and English strains. E. amylovora strains from central Europe and New Zealand had identical PFGE patters, as had strains from Egypt, Greece, and Turkey. PFGE of genomic DNA from American and English strains gave rise to dissimilar patterns. Patterns of some American strains resembled those from strains isolated in other parts of the world. The restriction fragment length polymorphisms observed by PFGE analysis can be used to group strains and may give hints about the course of distribution of the plant disease. From the sizes of the restriction fragments obtained, a molecular mass of approximately 4.5 Mb was calculated for the genome of E. amylovora.
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Database assignments for author(s): Klaus Geider

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Erwinia amylovora		France
	Erwinia amylovora		Greece
	Erwinia amylovora		Turkey
	Erwinia amylovora		United Kingdom
	Erwinia amylovora		New Zealand
	Erwinia amylovora		Egypt