Applied Entomology and Zoology (2018) 53, 463-474

Hisato Ishii, Yuya Mikawa, Yuko Murase, Shoji Sonoda, Norihide Hinomoto, Hidenari Kishimoto, Shingo Toyoshima and Masatoshi Toyama (2018)
Species composition and arthropod pest feeding of phytoseiid mites in a Japanese pear greenhouse
Applied Entomology and Zoology 53 (4), 463-474
Abstract: A population survey of spider mites and phytoseiid mites was conducted on Japanese pear leaves in a greenhouse. For the survey, the method to estimate phytoseiid mite species composition using quantitative sequencing was modified to be applicable for phytoseiid mite species inhabiting in the greenhouse. Results show the dominant appearance of Neoseiulus californicus (McGregor), Neoseiulus womersleyi (Schicha), and Neoseiulus makuwa (Ehara) from the end of June to late September and their contribution in spider mite control. PCR-based method to detect the ribosomal internal transcribed spacer (ITS) sequences of spider mites from phytoseiid mites was developed. The method shows sensitivity to detect the ITS sequences of Tetranychus urticae Koch from single N. californicus adult at 168 h after ingestion of the spider mite. PCR-based method to detect the cytochrome c oxidase subunit I sequences of several arthropod pests belonging to Hemiptera, Thysanoptera, and Acari from phytoseiid mites was also developed. Results show that phytoseiid mites prey on Eriophyes chibaensis (Kadono) and Aphis gossypii (Glover), in addition to spider mites.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Shoji Sonoda, Norihide Hinomoto, Shingo Toyoshima, Hidenari Kishimoto

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis gossypii	Pear (Pyrus)	Japan
	Tetranychus urticae	Pear (Pyrus)	Japan
	Eriophyes chibaensis	Pear (Pyrus)	Japan
Neoseiulus californicus (predator)	Tetranychus urticae	Pear (Pyrus)	Japan
Neoseiulus womersleyi (predator)			Japan
Neoseiulus makuwa (predator)			Japan